Metabarcoding as an effective complement of microscopic studies in revealing the composition of the diatom community – a case study of an oxbow lake of Tisza River (Hungary) with the description of a new Mayamaea species

Diatoms are valuable bioindicators and their traditional classification identification mainly based on the morphological characteristics of frustules. However, in recent years, DNA-based methods have been proposed rapidly growing scientific literature as a complementary tool to assess ecological status freshwaters. Diatom-based assessment uses indices calculated from sensitivity tolerance values well relative abundance species. Correct requires an accurate In present study, diatom assemblages oxbow lake were investigated using light scanning electron microscopy metabarcoding rbc L marker, results compared, intending match barcode sequences species that currently missing reference database. The is important wetland for bird conservation, although it impacted by land use. Taxon lists morphology considerably differed when bioinformatics analysis involved DADA2 pipeline with Diat.barcode Previously unknown sequence variants four pennate found additional BLAST search. Using phylogeny p-distance calculations could be matched three small-celled naviculoid under microscope. One them was new genus Mayamaea described species, ectorii . Additionally, spatial distribution maps several provided Hungarian territory.